Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation

Abstract Background Filamentous fungus Trichoderma reesei has been widely used as a workhorse for cellulase and xylanase productions. Xylanase reported the crucial accessory enzyme in degradation of lignocellulose higher accessibility cellulase. In addition, efficient hydrolysis xylan needs co-work multiple xylanolytic enzymes, which rise an increasing demand high yield biomass degradation. Results this study, hyper-producing system T. was established by tailoring two transcription factors, XYR1 ACE1, homologous overexpression major endo-xylanase XYNII. The expressed cocktail contained 5256 U/mL activity 9.25 ?-xylosidase ( p NPXase) activity. Meanwhile, level genes strain with overexpressed upregulated, well correlated amount XYR1-binding sites. expression associated enzymes would result more hydrolysis. Besides, 2310–3085 activities were achieved using soluble carbon source, economical than traditional strategy induction. Unexpectedly, deletion ace1 C30OExyr1 did not give any improvement, might be disturbed function complex formed between ACE1 XYR1. enzymatic alkali pretreated corn stover crude cocktails resulted 36.64% increase saccharification efficiency ratio vs FPase at 500, compared to that alone. Conclusions An platform developed RUT-C30. novel outstanding ability production greatly fit new perspective further engineering industrial purposes.